The 2-Minute Rule for cytometers

The 2-Minute Rule for cytometers

Blog Article

Data-enriched characterization of cell populations. Morphological details from pictures can include to your richness of flow cytometry data. By way of example, the determine exhibits an normally standard apoptosis assay using Annexin V and PI, incorporating cell imaging to characterize cells in Every single populace to expose morphologically distinctive attributes. These insights couldn't are actually obtained from multiplex staining on your own.

Irrespective of whether you’re a beginner or a professional, discover our resources to progress your investigate and knowledge in flow cytometry.

Incorporate acoustic focusing technologies with high-parameter Evaluation for versatile spectral unmixing and standard workflows.

The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a high-pace brightfield digital camera for simultaneous superior throughput flow cytometry and higher resolution brightfield imaging.

"An image is not very truly worth 1000 dots, but it surely genuinely helps to see visuals. It is excellent to view photos that correlate with staining. One example is, AnnexinV-positive cells which have been morphologically unique than balanced cells, or CD14+ monocytes that are much larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.

On top of that, it is illuminating to discover the number of doublets slip by way of regular singlet gates, and fantastic to verify the position of unpredicted double-beneficial activities in my Assessment."

Pair your Attune flow cytometer having an autosampler for enhanced workflow efficiency, and insert robotic integration for max throughput.

Constant image high quality even at large throughput Acoustic focusing in addition to a substantial-pace digital camera Incorporate to image these T cells persistently at very low or significant flow costs. Easily alter concentration and camera settings to fulfill experimental necessities.

Early signs of this contamination experienced in the beginning been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow check here Cytometer also allows you to use morphological features to find out exciting subpopulations that would not be evident from flow cytometry information by itself.

Consumers can perspective celebration photos in authentic-time to substantiate sample quality, improve protocols, and document the morphology of each and every mobile populace identified for Investigation. Imaging capture also Advantages from acoustic focusing, which really helps to place the cells so that a pointy, centered image is acquired.

Audio waves enable precise mobile alignment, causing Remarkable info quality and ~10X more quickly sample flow fees.

We Earlier shown the power of imaging Motor vehicle-T/Ramos cell interactions. Let’s examine just the population of best curiosity, the double optimistic activities, To find out more. We could now use prolonged morphological parameters (here, circularity and skewness of depth) to further study the characteristics of those populations and refine gating on these activities, raising data robustness.

Leverage the power of Highly developed know-how and innovation to accelerate your discoveries. Trust from the Attune Flow Cytometer family members to provide the reliability, velocity, and precision that established your function apart.

No clean/no lyse leukocyte Assessment with imaging. Cells have been obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Pro UX/UI enables end users to coach AI types for expanded programs. (A) The “Prepare Model” function offers annotation applications and genuine-time design teaching accuracy.

Gating on these prolonged parameters lets you swiftly and properly detect populations of fascination and make sure gating system with little or no guide evaluation. The Attune software features entry to condition-of-the-art device Understanding Investigation tools in an expertly developed consumer interface, which makes it exceptionally user friendly.

The Attune Xenith cytometer is really a high-parameter spectral analyzer, furnishing equally spectral unmixing and standard flow abilities for comprehensive and adaptable mobile Examination.

Agilent Benefit Guarantee assures you no less than 10 years of instrument use from the day you buy. Or else, We're going to credit rating you with the residual value of the system toward an upgraded design.

In this article, we present that by gating methods utilizing graphic-dependent quantitative parameters can distinguish interacting cells from coincident gatherings to a lot more accurately evaluate interacting cells.